Expose-R2 experment: Exploring Mars in low Earth orbit

The Expose-R2 experment on the outside of the Zvezda module of the International Space Station (ISS). 

Credit: DLR

In their quest to understand life's potential beyond Earth, astrobiologists study how organisms might survive in numerous environments, from the surface of Mars to the ice-covered oceans of Jupiter's moon, Europa.

For now, Earth is our only example of an inhabited planet, and studying the limits of habitability on Earth is a major component of astrobiology research.

For this reason, scientists collect data from places on our planet where life is pushed to the absolute limits of adaptability, from the Antarctic to the Arctic, and from smoldering thermal vents to highly acidic rivers.

But locations like the Antarctic Dry Valleys or deep-sea vents in the Pacific aren't the only places in which astrobiologists study life as we know it. Low Earth orbit provides an opportunity to observe Earth-life in the harsh conditions of space.

In the early hours of July 24th, 2014, a new astrobiology experiment began its journey from the Baikonur Cosmodrome in Kazakhstan to the International Space Station (ISS).

BIOMEX (Biology and Mars Experiment) launched onboard a Russian Progress cargo spacecraft and is one of four experiments that make up the EXPOSE-R2 facility, which will be mounted on the exterior of the ISS Zvezda module.

Just six hours after launch, the cargo ship successfully docked with the ISS.

Life on the Station
BIOMEX contains twelve different experimental packages that are designed to help determine life's potential on Mars.

The Institute of Planetary Research at the German Aerospace Center (DLR) is coordinating BIOMEX, but the project involves 25 participating institutions from around the world.

BIOMEX contains numerous chambers that are filled with biomolecules and organisms that include bacteria, archaea, algae, fungi, lichens and mosses.

Replicate samples spread across the compartments are subjected to a range of environmental conditions.

Some samples of each biomolecule or organism are embedded in a simulant Mars soil (ranging from just a single layer of soil to multiple layers), and other samples are left on their own to face the space environment without protection.

Various filters are also being used on the sample chambers to test exposure to different levels of radiation.

By doing this, scientists are able to simulate the solar radiation present at the martian surface. Some of the sample chambers are even pumped full of a simulated Mars atmosphere that is rich in carbon dioxide and pressurized to replicate conditions on Mars.

"To gain real insights into the behavior of biomolecules within a martian environment, we have to check the different parameters we might encounter on Mars," explained Dr. Jean-Pierre Paul de Vera of the German Aerospace Center (DLR) and the principle investigator for BIOMEX.

"This means we will approach, as much as possible on the ISS, martian conditions, including extreme temperature regimes, martian atmosphere by using Mars-like gases in the compartments of EXPOSE-R2, and the radiation regime, which we can never simulate in the labs on Earth."

The samples will spend up to one and a half years outside the space station, and the organisms inside will be monitored with temperature sensors and dosimeters, which monitor radiation exposure.

The goal is to see how exposure to these varied environmental pressures affects the survival of the organisms and the stability of important cellular components like membrane lipids, pigments, proteins and DNA.

The results of BIOMEX will help astrobiologists understand whether or not these biological materials can cope with conditions in the space environment and on Mars, and if being buried in martian soil might aid in their survival.

Tools for the Future
While the samples in BIOMEX are attached to the outside of the station, scientists on Earth will be working with replicate samples in the lab.

Here they will simulate martian conditions as best they can in the controlled environment of the laboratory and monitor the Earth-bound samples with a number of instruments.

View of a Progress vehicle connected to the Zvezda module of the ISS. 

The Zvezda Service Module was the first fully Russian contribution to the International Space Station. 

The module provides station living quarters, life support systems, electrical power distribution, data processing systems, flight control systems and propulsion systems. 

Credit: NASA

At the completion of the experiment, BIOMEX samples will be returned to Earth where scientists will take a close look at the results. In the laboratory, they will examine the stability of biomolecules after they have been exposed to the conditions in low Earth orbit.

This includes studying the signatures they leave behind in the sample chambers, which could be useful on future life-detection missions on Mars.

"BIOMEX is investigating the capacity of instruments to detect selected biosignatures (pigments, membrane composites, lipids etc.) in a Mars-like environment before and after space experiments, and also during Mars simulations in the lab," de Vera told reporters.

The set of spectroscopic instruments they are using on Earth are similar to those currently being eyed for Mars missions in the near future.

They include Raman, IR and UV/VIS spectroscopes. Initial tests in the lab have already turned up some interesting results.

Studies at the German Aerospace Center (DLR) in Cologne and Berlin indicate that biosignatures are altered by temperature and radiation.

This causes their appearance to differ from the signatures we normally observe in Earth conditions.

Beyond Astrobiology
Data from BIOMEX could also have some important applications beyond the realm of astrobiology according to Dr. de Vera.

Studying how biosignatures survive in a simulated Mars regolith might have lessons for archaeology experts on Earth who are looking for radiation-independent (e.g. not carbon 14-dating) methods to study ancient wooden objects.

In particular, the thermogravimetric methodology, which is used by de Vera and his team to test the bounded and remaining water in BIOMEX samples after they have faced the conditions of space, is of special interest for archaeologists.

Raman spectroscopy is also a technique that is growing in prominence for biological studies in numerous fields.

"Raman spectroscopy is used more and more in microbiology, pharmacology and medicine," said de Vera.

"The Robert Koch Institute in Berlin, which is cooperating with us, uses this method (coupled with other methods) to characterise microorganisms that can be harmful to health, and they have to be detected very fast to find out if there could be a risk of an epidemic."

The studies of biofilms in space could have some interesting implications for the health of astronauts and humans on Earth.

On Earth, biofilms are used in some health drinks to trigger the immune system. Studying biofilms in space can help determine whether or not these drinks might be safe for astronauts to consume in orbit, or if the space environment will cause biofilm cultures to rapidly mutate in such a way as to become harmful for consumption.

"Desiccation [removal of water] and radiation protection is also a very important issue," noted de Vera.

"Studies on the exposed samples might give more information about how the most resistant microorganisms are able to shield themselves efficiently, and which substances are responsible for their resistance.

The cosmetic and food industries are interested in these results."

In fact, the Fraunhofer Institute IZI for Cell Therapy and Immunology in Potsdam, Germany is already working with two of the organisms that de Vera and his team are studying.

One is a highly resistant cyanobacteria, and the other is a green algae. Thanks to BIOMEX, these organisms now have a home in low Earth orbit, clinging to the outside of the International Space Station.

The Mars Simulation Facility Laboratory. 

Credit: DLR

Further afield BIOMEX will help astrobiologists understand the potential for habitability on Mars.

If life ever originated on Mars, and if that life operated under the same biological principles as on Earth, could those organisms have adapted to survive on Mars in the present day?

By exploring this question, BIOMEX could help shape the future of Mars exploration, providing guidelines for where robotic explorers might search for signs of life on present-day Mars or signs of ancient life preserved in the regolith.

"With the data obtained by the selected biomolecules as potential biosignatures and which are exposed to the Mars-like conditions in space, we are building up a database that might have significant relevance for future exploration missions to Mars," said de Vera.

"This database might serve as back-up, or a systematically generated reference list that takes into account the martian environmental conditions that might influence the signatures of minerals, and possible fossils or biomolecules from potential extant life forms."

Russian Soyuz Foton-M Satellite fails to respond to commands

Soyuz engineers at Progress were trying to save a research satellite after it failed to respond to commands, which has left it in an improper orbit, Russian news agencies said.

The Foton-M satellite was launched on July 19 on a two-month mission to study the effect of weightlessness on plants and insects.

The satellite carries containers with living organisms, including five geckos, fruit flies and fungi, which are supposed to be jettisoned after two months in orbit and land in Russia.

There is no update on the status of the living cargo, since the containers are not designed to broadcast any telemetry while in space.

The mishap is the latest in series of setbacks that has plagued Russia's once-famed space programme.

Containers with biological experiment equipment being loaded into the Foton-M satellite. 

Photo by the Institute for Biomedical Problems. 

Fruiting Bodies: Dictyostelium discoideum

The fruiting bodies produced by the amoeba Dictyostelium discoideum as part of its reproductive cycle are pictured. Scientists have learned that some of the single-celled organisms display a primitive form of agriculture.

Instead of consuming all the bacteria at a particular site, they save some to 'seed' at other locations where food is scarce.

The amoeba species lives in slime moulds in the soil, where they exhibit an unusual form of social reproduction.

Dictyostelium chooses social over sexual reproduction whenever it runs out of food.

Fungi Can Quickly Mutate to Produce an Infectious Ability

Fungi have significant potential for "horizontal" gene transfer, a new study has shown, similar to the mechanisms that allow bacteria to evolve so quickly, become resistant to antibiotics and cause other serious problems.

This discovery, to be published March 18 in the journal Nature, suggests that fungi have the capacity to rapidly change the make-up of their genomes and become infectious to plants and possibly animals, including humans.

They are not nearly as confined to the more gradual processes of conventional evolution as had been believed, scientists say. And this raises issues not only for crop agriculture but also human health, because fungi are much closer on the "evolutionary tree" to humans than bacteria, and consequently fungal diseases are much more difficult to treat.

The genetic mechanisms fungi use to do this are different than those often used by bacteria, but the end result can be fairly similar. The evolution of virulence in fungal strains that was once believed to be slow has now been shown to occur quickly, and may force a renewed perspective on how fungi can behave, change and transfer infectious abilities.

"Prior to this we've believed that fungi were generally confined to vertical gene transfer or conventional inheritance, a slower type of genetic change based on the interplay of DNA mutation, recombination and the effects of selection," said Michael Freitag, an assistant professor of biochemistry and biophysics at Oregon State University.

"But in this study we found fungi able to transfer an infectious capability to a different strain in a single generation," he said. "We've probably underestimated this phenomenon, and it indicates that fungal strains may become pathogenic faster than we used to think possible."