Researchers from the London School of Hygiene and Tropical Medicine say mosquitoes are first deterred by the substance, but then later ignore it.
They say more research is needed to find alternatives to Deet, which was first developed by the US military.
The research was carried out on Aedes aegypti, a species of mosquito that spreads dengue and yellow fever.
The findings are published in the journal Plos One.
Dr James Logan from the London School of Hygiene and Tropical Medicine, said: "The more we can understand about how repellents work and how mosquitoes detect them, the better we can work out ways to get around the problem when they do become resistant to repellents."
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